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Description The BM8 monoclonal antibody reacts with mouse F4/80 antigen an approximately 160 kDa surface receptor It belongs to the EGF TM7 family of proteins As such it contains seven EGF like domains on its extracellular N terminus seven transmembrane spanning sequences and an intracellular C terminal domain showing homology to other

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Inhibitory role of the Bm8 protein in the propagation of

Lepidopteran nucleopolyhedroviruses have distinct viral tissue tropisms in host larvae We previously identified the Bm8 gene of Bombyx mori nucleopolyhedrovirus BmNPV the product of which inhibits viral propagation in the middle silk gland MSG However it is unknown whether this inhibitory function of the Bm8 protein is specific to MSGs.

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The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non destructive from destructive inflammation processes in the pancreas Furthermore it is a unique histological marker of the progression from peri insulitis to beta cell destruction and diabetes in a mouse diabetes model.

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Flow CytometryAnti F4/80 antibody BM8 ab16911 Overlay histogram showing HeLa cells stained with ab16911 red line The cells were fixed with methanol 5 min and incubated in 1x PBS 10 normal goat serum 0.3M glycine to block non specific protein protein interactions The cells were then incubated with the antibody ab16911 1/10

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F4/80 EMR1 is a heavily glycosylated G protein coupled receptor and is a well established marker for mouse macrophages 1 3 Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells 4 BM8.1 is widely used together with antibodies to CD115 c Fms CD11b and CD11c to identify myeloid/macrophage

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F4/80 BM8 is a rat monoclonal antibody raised against cultured bone marrow derived macrophages of mouse origin PRODUCT Each vial contains 100 µg IgG 2a in 1.0 ml of PBS with < 0.1 sodium azide and 0.1 gelatin F4/80 BM8 is available conjugated fluorescein sc 52664 FITC 100 tests in 2 ml for WB RGB IF IHC P and FCM APPLICATIONS

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Inhibitory role of the Bm8 protein in the propagation of

Lepidopteran nucleopolyhedroviruses have distinct viral tissue tropisms in host larvae We previously identified the Bm8 gene of Bombyx mori nucleopolyhedrovirus BmNPV the product of which inhibits viral propagation in the middle silk gland MSG However it is unknown whether this inhibitory fu

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F4/80 BM8.1 Rat mAb 71299 IF F4/80 BM8.1 ProLong Gold Antifade Reagent with DAPI #8961 # 71299S .

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B6 bm8 F 2 mice that were divided into H 2Kb/b H 2Kb/bm8 and H 2Kbm8/bm8 geno type groups 13 and challenged with the HVH 1 dose known to kill about 80 of B6 and about 10 of bm8 mice Fig 1A We reasoned that if the non mhc genes played a role in bm8 resistance one would expect similar survival rates in each of the groups

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F4/80 Antibody BM8 is a rat monoclonal IgG 2a cited in 72 publications provided at 100 µg/ml raised against cultured bone marrow derived macrophages of mouse origin recommended for detection of EMR1 of human origin F4/80 of mouse origin and the corresponding rat homolog by WB IF IHC P and FCM See F4/80 C 7 sc for F4/80

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Inhibitory role of the Bm8 protein in the propagation of

Bm8 disrupted BmNPV produced fewer BVs and more OBs than the wild type virus.Bm8 disruption accelerated the progression of viral infection in larval tissues. Bm8 disruption enhanced viral gene expression in BmNPV infected larval tissues. Bm8 protein suppresses viral propagation to varying degrees in each larval tissue.

Purified anti mouse F4/80 Antibody anti F4/80BM8

Description F4/80 is a 160 kD glycoprotein It is characterized as a member of the epidermal growth factor EGF transmembrane 7 TM7 family F4/80 also known as EMR1 or Ly71 has been widely used as a murine macrophage marker which is expressed on the majority of tissue macrophages including peritoneal macrophages macrophages in lung gut thymus and red pulp of spleen but not on the

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Adgre1 rat monoclonal antibody clone BM8 Biotin Product Data Clone Name BM8 Applications FC IF IHC Recommended Dilution Immunohistochemistry on Frozen Sections 1.0 µg/ml 1/200 Immunohistochemistry on Paraffin Sections not tested but work well with the purified antibody using Proteinase K pretreatment Recommended Positive Control Mouse spleen Has been described to